Video 12. Pollen tube interaction with a lre-5/lre-5 ovule that induces normal pollen tube reception in an in vitro guidance assay

Time-lapse imaging of LAT52:DsRed-tagged pollen tube entering a lre-5/lre-5 ovule, reaching DD3:GFP-marked synergid cell, and undergoing normal pollen tube reception.

The video shows merged time-lapse images captured in red and green channels.

Time elapsed between two frames is 10 minutes.

Ref: Tsukamoto, T., Qin Y., Huang, Y., Dunatunga, D. and Palanivelu, R. (2010). Plant J (in press).